Regitze Overgaard worked as an apprentice at goldsmith Ib Antvorksov in Gentofte in 1970, she finished her education as a goldsmith at the Copenhagen School of Arts and Crafts in 1973. In 1974 she established her own workshop in Copenhagen after having travelled to Russia, Italy, England and Yugoslavia. In 1974 she started designing for Andreas Mikkelsen. She collaborated with the jewellery company Buch + Deichmann from 1980-1986 and with Royal Copenhagen from 1976-1987. She started designing jewellery for Georg Jensen in 1987 and the collaboration is ongoing.

From 2010-2014 she worked as head of designs at Georg Jensen.

She has designed several collections for Georg Jensen: Magic Collection 2001, Infinity 2004, Carnival 2006, Zephyr 2006, Regitze silver 2009, Sphere 2010, Regitze gold 2011, Symbols 2012, Spirit 2013, Nocturne 2013, Magic line extension 2017 and she was responsible for reviving the jewellery lines Grape and Daisy in 2010.